Detection of two precursors to each of the subunits of human chorionic gonadotropin translated from placental mRNA in the wheat germ cell-free system.

@article{Godine1982DetectionOT,
  title={Detection of two precursors to each of the subunits of human chorionic gonadotropin translated from placental mRNA in the wheat germ cell-free system.},
  author={John E. Godine and William Chin and Joel F. Habener},
  journal={Biochemical and biophysical research communications},
  year={1982},
  volume={104 2},
  pages={
          463-73
        }
}
Abstract Polyadenylated RNA prepared from first trimester human placenta was translated in a membrane-free cell-free system derived from wheat germ. Analysis of the [ 35 S]methionine-labeled products by SDS-polyacrylamide electrophoresis demonstrated two proteins with apparent M r s of 14,500 and 16,000 that were specifically immunoprecipitated by antiserum to reduced and carboxylated bovine LH α , and two different proteins with apparent M r s of 18,500 and 21,000 that were specifically… CONTINUE READING