Detection of two orchid viruses using quartz crystal microbalance-based DNA biosensors.

@article{Eun2002DetectionOT,
  title={Detection of two orchid viruses using quartz crystal microbalance-based DNA biosensors.},
  author={Alvin Jin-Cherng Eun and Liqun Huang and F L M Chew and Sam Fong-Yau Li and S. -L. Wong},
  journal={Phytopathology},
  year={2002},
  volume={92 6},
  pages={654-8}
}
ABSTRACT We have developed a piezoelectric DNA-sensor based on DNA-RNA hybridization for the detection of two orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). Specific oligonucleotide probes modified with a mercaptohexyl group at the 5'-phosphate end were directly immobilized onto 10-MHz AT-cut quartz crystal microbalance (QCM). QCMs coated with such oligonucleotide probes were exposed to test solutions containing viral RNA for hybridization. Various… CONTINUE READING