Detection of two different mRNAs in a single section by dual in situ hybridization: a comparison between colorimetric and fluorescent detection.

@article{BarrosoChinea2007DetectionOT,
  title={Detection of two different mRNAs in a single section by dual in situ hybridization: a comparison between colorimetric and fluorescent detection.},
  author={Pedro Barroso-Chinea and Mar{\'i}a S. Aymerich and Mar{\'i}a M Castle and M{\'o}nica P{\'e}rez-Manso and Teresa Tu{\~n}{\'o}n and Elena Erro and Jos{\'e} L Lanciego},
  journal={Journal of neuroscience methods},
  year={2007},
  volume={162 1-2},
  pages={119-28}
}
We have compared the performance of two methods designed to simultaneously detect two different mRNAs within a single brain section by dual ISH. Specific mRNA riboprobes labeled with biotin and digoxigenin were simultaneously hybridized and visualized using either brightfield or fluorescence microscopy. For brightfield visualization, the biotin-labeled riboprobe was detected with a peroxidase chromogen, whereas, an alkaline phosphatase substrate was used for the detection of the digoxigenin… CONTINUE READING

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