Detection of two diatoms using sandwich hybridization integrated with nuclease protection assay (NPA-SH)

@article{Zhen2006DetectionOT,
  title={Detection of two diatoms using sandwich hybridization integrated with nuclease protection assay (NPA-SH)},
  author={Yu Zhen and Zhigang Yu and Qingsong Cai and Tiezhu Mi and Rongxiu Li},
  journal={Hydrobiologia},
  year={2006},
  volume={575},
  pages={1-11}
}
Nuclease protection assay (NPA) probes were designed to target the rRNA of Chaetoceros curvisetus and Skeletonema costatum, and quantitative sandwich hybridization integrated with nuclease protection assay (NPA-SH) was developed to detect C. curvisetus and S. costatum in culture and field samples in Jiaozhou Bay, China. The specificity and validity of the NPA-SH technique were tested with cultured pure strains, mixed strains and field samples, and by comparison with that of microscopy… CONTINUE READING