Detection of trypanosomatidPhytomonas parasitic in plants by polymerase chain reaction amplification of small subunit ribosomal DNA

@article{Teixeira1994DetectionOT,
  title={Detection of trypanosomatidPhytomonas parasitic in plants by polymerase chain reaction amplification of small subunit ribosomal DNA},
  author={Marta M. G. Teixeira and Marta Campaner and Erney Plessmann Camargo},
  journal={Parasitology Research},
  year={1994},
  volume={80},
  pages={512-516}
}
To improve the diagnosis ofPhytomonas infections in plants, we developed a polymerase chain reaction (PCR) assay using synthetic oligonucleotides complementary to conserved sequences of the 18S small subunit ribosomal (SSU) gene. From 10 ng upward of DNA of cultures ofPhytomonas isolated from plants, fruits, and insects, PCR amplified an 800-bp DNA band… CONTINUE READING