Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus.

@article{Paton1997DetectionOT,
  title={Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus.},
  author={David James Paton and Georgina Ibata and Jeffrey J Sands and Adrian McGoldrick},
  journal={Journal of virological methods},
  year={1997},
  volume={66 2},
  pages={303-9}
}
An RT-PCR method was developed that amplified genetic material from the 5' end of the S protein gene of both transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), but discriminated between the two by the size of the product generated. A number of restriction endonuclease enzymes were assessed for recognition of the amplicons so produced. The assay was shown to detect viral RNA from all of the 26 different TGEV and PRCV isolates examined, covering a period from… CONTINUE READING

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