Detection of toxigenic strains of Bacillus cereus and other Bacillus spp. with an improved cytotoxicity assay.

@article{Beattie1999DetectionOT,
  title={Detection of toxigenic strains of Bacillus cereus and other Bacillus spp. with an improved cytotoxicity assay.},
  author={S H Beattie and Andrew G. Williams},
  journal={Letters in applied microbiology},
  year={1999},
  volume={28 3},
  pages={221-5}
}
An improved qualitative cell cytotoxicity assay for the detection of Bacillus cereus emetic and enterotoxin is described. The presence of toxin in culture supernatant fluids was detected by measurement with the tetrazolium salt MTT, as it adversely affects the metabolic status of cultured CHO cells. Psychrotrophic B. cereus isolates (65) were assessed for toxin production using the cytotoxicity assay, and 91% of culture supernatant fluids were cytotoxic. Toxin assessment using BCET-RPLA and… CONTINUE READING

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