Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction.

@article{Fukuta2003DetectionOT,
  title={Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction.},
  author={Shiro Fukuta and Shinro Kato and Keiko Yoshida and Yuko Mizukami and Akira Ishida and Junnichi Ueda and Michio Kanbe and Yoshiyuki Ishimoto},
  journal={Journal of virological methods},
  year={2003},
  volume={112 1-2},
  pages={35-40}
}
The genomic DNA molecule of tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, was amplified from total DNA extracts of TYLCV-infected tomato (Lycopersicon esculentum) by the use of loop-mediated isothermal amplification (LAMP). The procedure was also used to amplify TYLCV DNA from total DNA extracts of individual whiteflies (Bemisia tabaci) that had fed on TYLCV-infected plants. One of the characteristics of the LAMP method is its ability to synthesize an extremely… CONTINUE READING

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