Detection of tick-borne encephalitis virus RNA in ticks (Ixodes ricinus) by the polymerase chain reaction.

@article{Ramelow1993DetectionOT,
  title={Detection of tick-borne encephalitis virus RNA in ticks (Ixodes ricinus) by the polymerase chain reaction.},
  author={C Ramelow and Jochen Suess and Dorothea Berndt and Michael Roggendorf and Eckart Schreier},
  journal={Journal of virological methods},
  year={1993},
  volume={45 1},
  pages={115-9}
}
A polymerase chain reaction for the detection of tick-borne encephalitis virus (TBEV) RNA in ticks was developed. Two pairs of primers for nested PCR were selected from the 5'-NCR and the 5'-terminus of the C protein coding region, which are highly conserved among the TBEV isolates sequenced so far. The sensitivity of the nested PCR was tested by dilution experiments of a TBEV positive brain suspension. The specificity of the PCR products was confirmed by Southern blotting. In a pilot study, 60… CONTINUE READING
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