Detection of the van alphabet and identification of enterococci and staphylococci at the species level by multiplex PCR.

@article{Depardieu2004DetectionOT,
  title={Detection of the van alphabet and identification of enterococci and staphylococci at the species level by multiplex PCR.},
  author={Florence Depardieu and Bruno 7 P{\'e}richon and Patrice Courvalin},
  journal={Journal of clinical microbiology},
  year={2004},
  volume={42 12},
  pages={5857-60}
}
A multiplex PCR assay was developed for detection of the six types of glycopeptide resistance characterized in enterococci and for identification of Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermidis at the species level. Primers targeting the genes vanA, vanB, vanC, vanD, vanE, vanG, and ddl of E. faecium and E. faecalis and nuc of S. aureus and a chromosomal portion specific to S. epidermidis were designed to allow amplification of fragments… CONTINUE READING
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Characterization of a divergent vanD-type resistance element from the first glycopeptide-resistant strain of Enterococcus faecium isolated in Brazil

  • L. M. Dalla Costa, P. E. Reynolds, H. A. Souza, D. C. Souza, M. F. Palepou, N. Woodford
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