Detection of the recombinant proteins in single transgenic microbial cell using laser tweezers and Raman spectroscopy.

@article{Xie2007DetectionOT,
  title={Detection of the recombinant proteins in single transgenic microbial cell using laser tweezers and Raman spectroscopy.},
  author={Changan Xie and Nhu N-Q Nguyen and Yong Zhu and Yong-qing Li},
  journal={Analytical chemistry},
  year={2007},
  volume={79 24},
  pages={9269-75}
}
Laser tweezers Raman spectroscopy (LTRS) has been used for the rapid detection of recombinant somatolactin protein produced in single Escherichia coli bacteria and Pichia pastoris yeast cell in the current study. A cDNA sequence encoding mature peptide of zebrafish somatolactin beta was inserted into two different expression vectors and transfected into E. coli or P. pastoris yeast cells. We measured Raman spectra of single E. coli cells at different culture times following the induction with… CONTINUE READING

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