Detection of the mycophenolate-inhibited form of IMP dehydrogenase in vivo.

@article{McPhillips2004DetectionOT,
  title={Detection of the mycophenolate-inhibited form of IMP dehydrogenase in vivo.},
  author={Christine C McPhillips and Judith W Hyle and Daniel Reines},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 33},
  pages={12171-6}
}
IMP dehydrogenase (IMPDH) is the rate-limiting enzyme for de novo GMP synthesis. Its activity is correlated with cell growth, and it is the target of a number of proven and experimental drug therapies including mycophenolic acid (MPA). MPA inhibits the enzyme by trapping a covalent nucleotide-enzyme intermediate. Saccharomyces cerevisiae has four IMPDH genes called IMD1-IMD4. IMD2 is transcriptionally regulated and is the only one that enables yeast to grow in the presence of MPA. We show here… CONTINUE READING