Detection of the free-living forms of sulfide-oxidizing gill endosymbionts in the lucinid habitat (Thalassia testudinum environment).

@article{Gros2003DetectionOT,
  title={Detection of the free-living forms of sulfide-oxidizing gill endosymbionts in the lucinid habitat (Thalassia testudinum environment).},
  author={Olivier Gros and Martine Liberge and Abdelaziz Heddi and Chaqu{\'e} Khatchadourian and Horst Felbeck},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 10},
  pages={6264-7}
}
Target DNA from the uncultivable Codakia orbicularis endosymbiont was PCR amplified from sea-grass sediment. To confirm that such amplifications originated from intact bacterial cells rather than free DNA, whole-cell hybridization (fluorescence in situ hybridization technique) with the specific probe Symco2 was performed along with experimental infection of aposymbiotic juveniles placed in contact with the same sediment. Taken together, the data demonstrate that the sulfide-oxidizing gill… CONTINUE READING

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