Detection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.

@article{Peter2000DetectionOT,
  title={Detection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.},
  author={Trevor F. Peter and Anthony F. Barbet and Arthur R. Alleman and Biggy H. Simbi and Michael J. Burridge and Sunita Mahan},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 4},
  pages={1539-44}
}
We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR assay and applied it to field ticks. The assay detected DNA of 37 isolates of C. ruminantium originating from sites throughout the distribution of heartwater and had a specificity… CONTINUE READING
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