Detection of the JAK2(V617F) mutation in myeloproliferative disorders by melting curve analysis using the LightCycler system.


CONTEXT A specific mutation, JAK2(V617F), was recently recognized as having diagnostic value for myeloproliferative disorders. No practical assay is currently available for routine use in a clinical laboratory. OBJECTIVE We report the development of a real-time polymerase chain reaction melting curve analysis assay that is appropriate for molecular… (More)


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