Detection of tetramerization domains in vivo by cooperative DNA binding to tandem lambda operator sites.

Abstract

Chimeric proteins comprising the N-terminal DNA binding domain of lambda repressor fused to a fragment of a foreign protein have been used to detect oligomerization of the latter. Fusions containing dimeric and tetrameric leucine zipper domains can be distinguished based on their in vivo repressor activities on a pair of cat-lacZ reporter strains. Repressor… (More)

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Cite this paper

@article{Zeng1997DetectionOT, title={Detection of tetramerization domains in vivo by cooperative DNA binding to tandem lambda operator sites.}, author={Xiaofeng Zeng and Jan C.-C. Hu}, journal={Gene}, year={1997}, volume={185 2}, pages={245-9} }