Detection of superoxide anion generation by equine spermatozoa.

  title={Detection of superoxide anion generation by equine spermatozoa.},
  author={Khalida Sabeur and Barry A Ball},
  journal={American journal of veterinary research},
  volume={67 4},
OBJECTIVE To identify the generation of the superoxide anion by equine spermatozoa. SAMPLE POPULATION Multiple ejaculates collected from 3 Thoroughbred stallions. PROCEDURES Induced superoxide production by reduced nicotinamide adenine dinucleotides (NAD[P]H; ie, reduced nicotinamide adenine dinucleotide [NADH] and reduced nicotinamide adenine dinucleotide phosphate [NADPH]) was measured by use of a nitroblue tetrazolium (NBT) reduction assay on whole spermatozoa and a cytochrome c… CONTINUE READING
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