Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels

@inproceedings{Atkin2010DetectionOS,
  title={Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels},
  author={Janus W Atkin and Alan Radford and Karen P Coyne and Jenny Stavisky and Julian C Chantrey},
  booktitle={BMC veterinary research},
  year={2010}
}
BACKGROUND Squirrel poxvirus (SQPV) is highly pathogenic to red squirrels (Sciurus vulgaris), and is a significant contributing factor to the local extinction of the species in most parts of England and Wales, where infection is endemic in Eastern grey squirrel (Sciurus carolinensis) populations. Although a nested PCR assay has been used successfully to study the epidemiology of SQPV, samples have a long processing time and the assay is not quantifiable. RESULTS This project describes the… CONTINUE READING
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