Detection of specific sequences among DNA fragments separated by gel electrophoresis.

@article{Southern1975DetectionOS,
  title={Detection of specific sequences among DNA fragments separated by gel electrophoresis.},
  author={Edwin M. Southern},
  journal={Journal of molecular biology},
  year={1975},
  volume={98 3},
  pages={
          503-17
        }
}
  • E. Southern
  • Published 1975
  • Biology, Medicine
  • Journal of molecular biology
This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters. The fragments can then be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography. The method is illustrated by analyses of restriction fragments complementary to ribosomal RNAs from Escherichia coli and Xenopus laevis , and from several mammals. 
Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose.
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Genomic DNA that has been digested with a restriction endonuclease and fractionated by electrophoresis on an agarose gel can be recovered on glass-fiber filters by a new blotting scheme, useful in the analysis of complex genomes and in the isolation and cloning of particular sequences. Expand
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Vacuum transfer of DNA to filters for detecting interindividual polymorphism by Southern's blotting hybridization method
TLDR
The data are provided on the possibility of detecting interindividual polymorphism from the organization of repeated DNA sequences of the alphoid type in human genome on the basis of the Sothern blotting hybridization method. Expand
Spot blot: a hybridization assay for specific DNA sequences in multiple samples.
TLDR
Small aliquots of DNA-containing samples from gradient or column fractions are spotted onto agarose plates containing ethidium bromide, and the DNA is visualized qualitatively, and transferred to nitrocellulose filters for hybridization. Expand
Southern Blotting and Related DNA Detection Techniques
Southern blotting is a technique for transfer of DNA molecules from an electrophoresis gel to a nitrocellulose or nylon membrane, and is carried out prior to detection of specific molecules byExpand
An easy and efficient procedure for the isolation of pure DNA restriction fragments from agarose gels.
TLDR
It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. Expand
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  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1974
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