Detection of specific DNA lesions by a combination of comet assay and FISH in plants.

@article{Menke2000DetectionOS,
  title={Detection of specific DNA lesions by a combination of comet assay and FISH in plants.},
  author={Matthew Menke and Karel J. Angelis and Ingo Schubert},
  journal={Environmental and molecular mutagenesis},
  year={2000},
  volume={35 2},
  pages={132-8}
}
We have studied comet formation on Vicia faba nuclei embedded in agarose and treated with the endonucleases DNase I (to produce SSBs or DSBs at random sites), FokI (to produce DSBs preferentially within FokI repeats), or EcoRI (to produce DSBs at random sites but not within FokI elements). DNase I-induced SSBs were detected when enzyme treatment was… CONTINUE READING