Detection of site-specific proteolysis in secretory pathways.

@article{Kim2002DetectionOS,
  title={Detection of site-specific proteolysis in secretory pathways.},
  author={Sung Yun Kim and Daeho Park and Myungsok Oh and Saravanan Sellamuthu and Woo Jin Park},
  journal={Biochemical and biophysical research communications},
  year={2002},
  volume={296 2},
  pages={419-24}
}
We report here a genetic assay suitable for detecting site-specific proteolysis in secretory pathways. The yeast enzyme invertase is linked to the truncated lumenal region of the yeast Golgi membrane protein STE13 via a protease substrate domain in a Saccharomyces cerevisiae strain lacking invertase. When the substrate is cleaved by a specific protease, the invertase moiety is released into the periplasmic space where it degrades sucrose to glucose and fructose. Therefore, site-specific… CONTINUE READING

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