Detection of single quantum dots in model organisms with sheet illumination microscopy.

@article{Friedrich2009DetectionOS,
  title={Detection of single quantum dots in model organisms with sheet illumination microscopy.},
  author={Mike Friedrich and Revaz Nozadze and Qiang Gan and Monika Zelman-Femiak and Vladimir Ermolayev and Toni Ulrich Wagner and Gregory S. Harms},
  journal={Biochemical and biophysical research communications},
  year={2009},
  volume={390 3},
  pages={722-7}
}
Single-molecule detection and tracking is important for observing biomolecule interactions in the microenvironment. Here we report selective plane illumination microscopy (SPIM) with single-molecule detection in living organisms, which enables fast imaging and single-molecule tracking and optical penetration beyond 300 microm. We detected single nanocrystals in Drosophila larvae and zebrafish embryo. We also report our first tracking of single quantum dots during zebrafish development, which… CONTINUE READING
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