Detection of single oxygen molecules with fluorescence-labeled hemocyanins.


This study introduces a method to detect individual oxygen molecules by fluorescence microscopy of single hemocyanins. These respiratory proteins from a tarantula bind oxygen with high affinity. A spectrometric signature of the oxygenated protein is transferred to an attached fluorescence label, which can be detected at the single-molecule level. This technique opens new perspectives for the development of small and sensitive oxygen sensors as well as for the investigation of cooperative oxygen binding in respiratory proteins.

Cite this paper

@article{Erker2005DetectionOS, title={Detection of single oxygen molecules with fluorescence-labeled hemocyanins.}, author={Wolfgang Erker and Sven Sdorra and Thomas Basch{\'e}}, journal={Journal of the American Chemical Society}, year={2005}, volume={127 42}, pages={14532-3} }