Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes.

@article{Talia2011DetectionOS,
  title={Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes.},
  author={Paola M Talia and Eduardo Jos{\'e} Greizerstein and Horacio Esteban Hopp and Norma Paniego and Lidia Poggio and Ruth Amalia Heinz},
  journal={Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al},
  year={2011},
  volume={35 1},
  pages={19-28}
}
Bacterial artificial chromosome-fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for… CONTINUE READING