Detection of serum alkaline phosphatase isoenzymes with phenolphthalein monophosphate following cellulose acetate electrophoresis.

Abstract

Serum containing normal and abnormal levels of alkaline phosphatase activity were assayed for total enzyme activity, then fractionated by electrophoresis on cellulose acetate membranes for 20 mm. The new substrate, phenolphthalein monophosphate, was employed to locate the isoenzymes on the cellulose acetate membranes and to measure their activity by eluting… (More)

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