Detection of scrapie-associated fibrils (SAF) and SAF proteins from scrapie-affected sheep.

  title={Detection of scrapie-associated fibrils (SAF) and SAF proteins from scrapie-affected sheep.},
  author={Richard Rubenstein and Patricia A. Merz and Richard J. Kascsak and Richard I. Carp and Calogero Scalici and C L Fama and Henryk M. Wiśniewski},
  journal={The Journal of infectious diseases},
  volume={156 1},
Scrapie-associated fibrils (SAF) were detected by negative-stain electron microscopy in the brains (by two different isolation procedures) and spleens of sheep naturally and experimentally infected with scrapie. Although the numbers of SAF varied from case to case, the yield of SAF from brains of naturally affected sheep was lower than that from experimentally affected sheep. SAF-specific, protease-resistant proteins (PrPs) were detected by silver staining and western blot analysis in most… CONTINUE READING
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