Detection of rifampin-resistant Mycobacterium tuberculosis in sputa by nested PCR-linked single-strand conformation polymorphism and DNA sequencing.

@article{Kim2001DetectionOR,
  title={Detection of rifampin-resistant Mycobacterium tuberculosis in sputa by nested PCR-linked single-strand conformation polymorphism and DNA sequencing.},
  author={Beom Joon Kim and Kwan Hoon Lee and Bo Ya Na Park and Eun Mi Park and Yong Gyu Park and Gill Han Bai and Sun Joo Kim and Yoon Hoh Kook},
  journal={Journal of clinical microbiology},
  year={2001},
  volume={39 7},
  pages={2610-7}
}
Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis. However, due to the nonspecific amplification of rpoB DNA from nontuberculous mycobacteria these methods cannot be directly applied to clinical specimens such as sputa. We developed a nested PCR method that can specifically amplify the rpoB DNA of… CONTINUE READING