Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria.

  title={Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria.},
  author={Shannon R Mackey and Jayna L. Ditty and Eugenia M Clerico and Susan S Golden},
  journal={Methods in molecular biology},
The unicellular cyanobacterium Synechococcus elongatus PCC 7942 is the model organism for studying prokaryotic circadian rhythms. Although S. elongatus does not display an easily measurable overt circadian behavior, its gene expression is under circadian control; hence, a "behavior" is created by linking a cyanobacterial promoter to either the bacterial luxAB or firefly luc luciferase genes to create reporter fusions whose activity can be easily monitored by bioluminescence. Numerous vectors… CONTINUE READING

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