Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction.

@article{Perez2011DetectionOR,
  title={Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction.},
  author={Jonas W Perez and Elizabeth Vargis and Patricia K. Russ and Frederick R Haselton and David D. Wright},
  journal={Analytical biochemistry},
  year={2011},
  volume={410 1},
  pages={141-8}
}
In traditional immuno-polymerase chain reaction (immuno-PCR), a single antibody recognition event is associated with one to three DNA tags, which are subsequently amplified by PCR. Here we describe a nanoparticle-amplified immuno-PCR (NPA-IPCR) assay that combines antibody recognition of enzyme-linked immunosorbent assay (ELISA) with a 50-fold nanoparticle valence amplification step prior to tag amplification by PCR. The assay detects a respiratory syncytial virus (RSV) surface protein using an… CONTINUE READING

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