Detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification.


Red-spotted grouper nervous necrosis virus (RGNNV) causes high mortality in marine fish larvae cultured in China. To control better an outbreak of this virus, a rapid, specific and sensitive detection method based on loop-mediated isothermal amplification (LAMP) was developed. A set of four primers, two outer and two inner, was designed from RGNNV genome… (More)
DOI: 10.1016/j.jviromet.2009.09.009