Detection of recombinant growth hormone in human plasma by a 2‐D PAGE method

@article{Kohler2008DetectionOR,
  title={Detection of recombinant growth hormone in human plasma by a 2‐D PAGE method},
  author={Maxie Kohler and Klaus P{\"u}schel and Dimitri Sakharov and Alexander Tonevitskiy and Wilhelm Schänzer and Mario Thevis},
  journal={ELECTROPHORESIS},
  year={2008},
  volume={29}
}
Human growth hormone (GH) has several central metabolic functions including bone growth in childhood, and its anabolic and lipolytic effects in particular are assumed reasons for the abuse of GH by athletes. Human endogenous GH consists of a main 22 kDa variant and several isoforms. In contrast, recombinant GH consists of only one variant being identical to the main endogenous isoform. The method presented here separates different isoforms by 2‐D PAGE after isolation of GH from plasma using an… Expand
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