Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe

@article{Luo2006DetectionOR,
  title={Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe},
  author={Ji-dung Luo and Err-Cheng Chan and Chun-Liang Shih and Tai-long Chen and Ying Liang and Tsann-Long Hwang and Chiuan-Chian Chiou},
  journal={Nucleic Acids Research},
  year={2006},
  volume={34},
  pages={e12 - e12}
}
The major problem of using somatic mutations as markers of malignancy is that the clinical samples are frequently containing a trace amounts of mutant allele in a large excess of wild-type DNA. Most methods developed thus far for the purpose of tickling this difficult problem require multiple procedural steps that are laborious. We report herein the development of a rapid and simple protocol for detecting a trace amounts of mutant K-ras in a single tube, one-step format. In a capillary PCR, a… CONTINUE READING

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