Detection of pyrogenicity on medical grade polymer materials using rabbit pyrogen, LAL and ELISA method.

@article{Mohanan2011DetectionOP,
  title={Detection of pyrogenicity on medical grade polymer materials using rabbit pyrogen, LAL and ELISA method.},
  author={P. V. Mohanan and Siddharth Banerjee and Chandrika S. Geetha},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2011},
  volume={55 5},
  pages={1170-4}
}
The objective of the study is to detect the pyrogenicity of five medical grade gelatinous polymer materials, intended for the manufacturing of capsule for pharmaceutical applications, by an indigenously developed ELISA, LAL and rabbit pyrogen assays. The ELISA methodology includes the incubation of the sample extract with blood from a healthy donor at 37°C. Any pyrogen present in the extract induces the IL-1β which can be determined by ELISA. The rabbit pyrogen and LAL assays were performed as… CONTINUE READING

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