Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction.

@article{Slots1995DetectionOP,
  title={Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction.},
  author={J\orgen Slots and A Ashimoto and Mi hael J. Flynn and Guo-li Li and Casey Chen},
  journal={Clinical infectious diseases : an official publication of the Infectious Diseases Society of America},
  year={1995},
  volume={20 Suppl 2},
  pages={S304-7}
}
The utility of the 16S ribosomal RNA-based polymerase chain reaction (PCR) for detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Campylobacter rectus, Eikenella corrodens, Porphyromonas gingivalis, Prevotella intermedia, and Treponema denticola was examined and compared with that of anaerobic culture. Primer pairs consisting of 20-27 nucleotides amplified 404- to 688-bp regions of 16S ribosomal RNA genes of these organisms. This method had a lower detection limit of 50… CONTINUE READING