Detection of protein tyrosine phosphorylation by open sandwich fluoroimmunoassay.

  title={Detection of protein tyrosine phosphorylation by open sandwich fluoroimmunoassay.},
  author={Yoshiyuki Sasajima and Takahide Aburatani and Kenzo Sakamoto and Hiroshi Ueda},
  journal={Biotechnology progress},
  volume={22 4},
In the era of proteomics, high-throughput screening of posttranslational modification states of proteins, especially protein phosphorylation, is considered of utmost importance. However, current protein phosphorylation detection methods depend on either the combination of proteolysis and mass spectrometry, or time-consuming immunoassay that requires inevitable washing processes. As a way to rapidly assay protein phosphorylation events, here we propose the use of Open Sandwich immunoassay that… CONTINUE READING