Detection of protein-protein interactions using tandem affinity purification.

Abstract

Tandem affinity purification (TAP) is an invaluable technique for identifying interaction partners for an affinity tagged bait protein. The approach relies on the fusion of dual tags to the bait before separate rounds of affinity purification and precipitation. Frequently two specific elution steps are also performed to increase the specificity of the… (More)
DOI: 10.1007/978-1-4939-1034-2_10

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