Detection of protein-protein interactions among lens crystallins in a mammalian two-hybrid system assay.

@article{Fu2002DetectionOP,
  title={Detection of protein-protein interactions among lens crystallins in a mammalian two-hybrid system assay.},
  author={Ling Lin Fu and Jack J. N. Liang},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 6},
  pages={4255-60}
}
alpha-Crystallin consists of two subunits, alphaA and alphaB, and each can form an oligomer by itself or with the other. The aggregation arises from interdomain interactions. However, it is not known whether such interactions also exist among alpha-, beta-, and gamma-crystallins. This heterogeneous crystallin interaction is far weaker than the homogeneous crystallin interaction and is difficult to detect by conventional spectroscopic measurements. We used a mammalian two-hybrid system in this… CONTINUE READING

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