• Corpus ID: 33855860

Detection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples.

@article{Kittigul2000DetectionOP,
  title={Detection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples.},
  author={Leera Kittigul and Boonyos Raengsakulrach and Sontana Siritantikorn and Raweewan Kanyok and Fuangfa Utrarachkij and Pornphan Diraphat and Vipa Thirawuth and Kanokrat Siripanichgon and S Pungchitton and Kitja Chitpirom and N Chaichantanakit and K Vathanophas},
  journal={The Southeast Asian journal of tropical medicine and public health},
  year={2000},
  volume={31 1},
  pages={
          41-6
        }
}
A modified adsorption-elution technique for concentration of enteric viruses from sewage and water samples was developed. The viruses in water were concentrated by negatively charged membrane filtration, eluted with 2.9% tryptose phosphate broth containing 6% glycine pH 9.0, and reconcentrated using centrifugation by a speedVac concentrator. The presence of poliovirus, hepatitis A virus (HAV) RNA, and rotavirus antigen was determined by cell culture isolation, nested polymerase chain reaction… 

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