• Corpus ID: 33855860

Detection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples.

@article{Kittigul2000DetectionOP,
  title={Detection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples.},
  author={Leera Kittigul and Boonyos Raengsakulrach and Sontana Siritantikorn and Raweewan Kanyok and Fuangfa Utrarachkij and Pornphan Diraphat and Vipa Thirawuth and Kanokrat Siripanichgon and S Pungchitton and Kitja Chitpirom and N Chaichantanakit and K Vathanophas},
  journal={The Southeast Asian journal of tropical medicine and public health},
  year={2000},
  volume={31 1},
  pages={
          41-6
        }
}
A modified adsorption-elution technique for concentration of enteric viruses from sewage and water samples was developed. The viruses in water were concentrated by negatively charged membrane filtration, eluted with 2.9% tryptose phosphate broth containing 6% glycine pH 9.0, and reconcentrated using centrifugation by a speedVac concentrator. The presence of poliovirus, hepatitis A virus (HAV) RNA, and rotavirus antigen was determined by cell culture isolation, nested polymerase chain reaction… 
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An efficient virus concentration method and RT-nested PCR for detection of rotaviruses in environmental water samples.
An improved method for concentrating rotavirus from water samples.
TLDR
Reconcentration of water samples by a speedVac concentrator showed significantly higher rotavirus recovery than polyethylene glycol precipitation through both negatively and positively charged filters (p-value <0.001).
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The study shows that the high viral load in sewage results in a discharge to the environment of a large amount of virus despite sewage treatment, and the advantage of a more advanced treatment is demonstrated.
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A significant presence of HAV and bacterial indicators of fecal contamination in raw oysters, which are a health risk for consumers and a source of gastrointestinal illness, are suggested.
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The results revealed that eight PV strains detected in the present study two of each were PV1 and PV2, and four were PV3 serotypes of the Sabin vaccine strains, demonstrating the presence of PV1, PV2 and PV3 Sabin vaccines strains in children with acute gastroenteritis in Chiang Mai, Thailand.
Enteric Viruses And Aquatic Environment
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Traditional cell culture infectivity assay and molecular biology tools like nucleic acid amplification, microarray based assay or combination of cell culture and polymerase chain reaction are used as valuable informative tool to detect enteric viruses in aquatic environment.
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