Detection of phospholipid phase separation. A multifrequency phase fluorimetry study of 1,6-diphenyl-1,3,5-hexatriene fluorescence.

  title={Detection of phospholipid phase separation. A multifrequency phase fluorimetry study of 1,6-diphenyl-1,3,5-hexatriene fluorescence.},
  author={T. Parasassi and F. Conti and M. Glaser and E. Gratton},
  journal={The Journal of biological chemistry},
  volume={259 22},
Using multifrequency phase and modulation fluorometry and a nonlinear least-squares analysis of lifetime data, we were able to determine the complex decay of 1,6-diphenyl-1,3,5-hexatriene (DPH) in synthetic phospholipid bilayers. Our results showed a monoexponential decay of DPH in the pure isotropic solvents studied, over a wide temperature range, and a double-exponential decay of DPH in phospholipids, both above and below the transition. During the transition, and in mixed-phase phospholipids… Expand
Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane.
The fluorescence decay of 1,6 diphenyl-1,3,5-hexatriene (DPH) has been used to characterize aspects of the erythrocyte membrane structure related to the microheterogeneity of the lipid bilayer, suggesting a homogenizing effect of cholesterol. Expand
Fluorescence dynamics of diphenyl-1,3,5-hexatriene-labeled phospholipids in bilayer membranes.
A comparative study of the dynamical fluorescence properties of three phosphatidylcholines having a diphenyl-1,3,5-hexatriene (DPH) group attached at different depths from the head group incorporated into membrane vesicles has been carried out, confirming previous data in the literature. Expand
Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene in phospholipid vesicles.
It is proposed that the lifetime distribution is generated by a continuum of different environments of the DPH molecule characterized by different dielectric constants below the transition temperature in the gel phase, and above the transition, in the liquid-crystalline phase, the translational and rotational mobility of theDPH molecule increases, and the D PH experiences an average environment during the excited-state lifetime. Expand
Membranes modification of differentiating proerythroblasts. Variation of 1,6-diphenyl-1,3,5-hexatriene lifetime distributions by multifrequency phase and modulation fluorimetry.
The fluorescence emission of 1,6-diphenyl-1,3,5-hexatriene (DPH) in K562 cell membranes has been studied using multifrequency phase and modulation fluorimetry and marked variations were observed during the four initial days of the erythroid differentiation. Expand
Phosphatidic acid affects structural organization of phosphatidylcholine liposomes. A study of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammonium-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) fluorescence decay using distributional analysis.
The fluorescence decay of TMA-DPH was used to study micro-heterogeneity of 1,2-dimyristoyl-3-sn-phosphatidylcholine liposomes and to characterize the effect of phosphatidic acid on the correlation between fluorescence microheterogeneity and membrane permeability. Expand
Lateral distribution of a pyrene-labeled phosphatidylcholine in phosphatidylcholine bilayers: fluorescence phase and modulation study.
The results indicate that PyrPC is randomly distributed in pure gel and fluid phosphatidylcholine bilayers. Expand
Orientational distribution of 1,6-diphenyl-1,3,5-hexatriene in phospholipid vesicles as determined by global analysis of frequency domain fluorimetry data.
The results suggest that a single population of DPH molecules was present in the bilayers with their orientational distributions dependent upon the physical state of the membranes in the pure phases. Expand
Variation of 1,6-Diphenyl-1,3,5-Hexatriene Lifetime Distributions in Differentiating Proerythroblasts. A Multifrequency Phase and Modulation Fluorometry Study
During the cell differentiation process structural and functional properties peculiar to the mature, specialized cell are acquired. Although the molecular basis of the process are still largelyExpand
Analysis of cell membrane micro-heterogeneity using the fluorescence lifetime of DPH-type fluorophores.
It is concluded that in certain circumstances information pertaining to the protein-lipid interfacial region of membranes can be extracted from fluorescence decay heterogeneity properties. Expand
A dimerization model for the concentration dependent photophysical properties of diphenylhexatriene and its phospholipid derivatives. DPHpPC and DPHpPA.
It is concluded that the dimerization model allows rationalization of many of the anomalous photophysical properties of DPH and its derivatives in membranes. Expand


Biochemistry 20,4257-4262 chemistry
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Proceedings from the NATO-ASZ Physical Methods on Biological Membranes
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Chem . 252 , 2163 - 2169 ( 1981 ) Biochemistry 20 , 4257 - 4262 chemistry
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