Detection of phagocyte-derived free radicals with spin trapping techniques: effect of temperature and cellular metabolism.

@article{Rosen1988DetectionOP,
  title={Detection of phagocyte-derived free radicals with spin trapping techniques: effect of temperature and cellular metabolism.},
  author={Gerald Rosen and Bradley E. Britigan and Michael S. Cohen and S P Ellington and Michael J. Barber},
  journal={Biochimica et biophysica acta},
  year={1988},
  volume={969 3},
  pages={236-41}
}
Human neutrophils activated with either particulate or soluble stimuli generate oxygen-centered free radicals which are detected by spin trapping in conjunction with electron spin resonance (ESR) spectroscopy. We investigated the effect of temperature on ESR spectra resulting from stimulation of human neutrophils with phorbol myristate acetate (PMA) or… CONTINUE READING