Detection of peptidoglycan and endotoxin in dialysate, using silkworm larvae plasma and limulus amebocyte lysate methods.

@article{Tsuchida1997DetectionOP,
  title={Detection of peptidoglycan and endotoxin in dialysate, using silkworm larvae plasma and limulus amebocyte lysate methods.},
  author={Kenji Tsuchida and Yoshiaki Takemoto and Satoru Yamagami and H. Edney and Maho Niwa and Mitsukini Tsuchiya and Tadamitsu Kishimoto and Stanley Shaldon},
  journal={Nephron},
  year={1997},
  volume={75 4},
  pages={
          438-43
        }
}
Silkworm larvae plasma (SLP) reagent is activated by peptidoglycan (PG), a fragment of both the gram-positive and gram-negative bacterial cell wall, as well as beta-glucan (BG), a component of fungi. It is possible to measure contamination of gram-positive bacteria quantitatively by combining the conventional limulus amebocyte lysate (LAL) and PG measurement methods. Therefore, a more highly accurate analysis of dialysate can be made using both SLP and LAL methods to detect endotoxin (ET) and… CONTINUE READING

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