Detection of pathogenic leptospires in animals by PCR based on lipL21 and lipL32 genes.

@article{Cheemaa2007DetectionOP,
  title={Detection of pathogenic leptospires in animals by PCR based on lipL21 and lipL32 genes.},
  author={P S Cheemaa and Sameer Srivastava and R. Amutha and Sandeep Singh and Harsharan Pal Singh and Maninder Sandey},
  journal={Indian journal of experimental biology},
  year={2007},
  volume={45 6},
  pages={568-73}
}
Efficacy of primers capable of amplifying conserved outer membrane protein (OMP) genes i.e., lipL21 and lipL32 of Leptospira strains was tested for rapid and early diagnosis of the leptospirosis using a polymerase chain reaction (PCR). These OMP genes were found to be conserved in various leptospiral serovars viz., Canicola, Pomona, Icterohaemorrhagiae, Pyrogenes, Sejroe, Grippotyphosa, Ballum and Tarassovi as PCR products of 561 bp and 756 bp were obtained by PCR employing lipL21 and lipL32… CONTINUE READING