Detection of ovine antibody to Brucella ovis by indirect enzyme immunoassay.

@article{Nielsen2007DetectionOO,
  title={Detection of ovine antibody to Brucella ovis by indirect enzyme immunoassay.},
  author={Klaus Nielsen and Patrick H. Smith and Wei Ling Yu and Ximena Rojas and B{\'a}rbara P{\'e}rez and Susana Conde and Luis Ernesto Samartino and Claudia Robles},
  journal={Journal of immunoassay & immunochemistry},
  year={2007},
  volume={28 3},
  pages={243-50}
}
Because some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. An early version of the assay gave a performance index (PI=sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. This assay used RLPS from B. ovis as the antigen and a… CONTINUE READING