Detection of novel influenza A(H1N1) virus by real-time RT-PCR.

@article{Whiley2009DetectionON,
  title={Detection of novel influenza A(H1N1) virus by real-time RT-PCR.},
  author={David M. Whiley and S Bialasiewicz and Cheryl Bletchly and Cassandra E. Faux and Bruce J Harrower and Allan R. Gould and Stephen Bernard Lambert and Graeme R. Nimmo and Michael D. Nissen and Theo P. Sloots},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2009},
  volume={45 3},
  pages={
          203-4
        }
}
Accurate and rapid diagnosis of novel influenza A(H1N1) infection is critical for minimising further spread through timely implementation of antiviral treatment and other public health based measures. In this study we developed two TaqMan-based reverse transcription PCR (RT-PCR) methods for the detection of novel influenza A(H1N1) virus targeting the haemagglutinin and neuraminidase genes. The assays were validated using 152 clinical respiratory samples, including 61 Influenza A positive… CONTINUE READING

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