Detection of nonsense and frameshift mutations in BRCA1 gene using a new plasmid vector pPhoA-frame

Abstract

The technique for detecting frameshift and nonsense mutations in the human BRCA1 gene has been suggested. The technique presumes the construction of recombinant plasmids where the tested DNA fragment is placed in frame with alkaline phosphatase gene of Escherichia coli (phoA). A special plasmid pPhoA-frame was constructed for this analysis, and the plasmid… (More)
DOI: 10.1134/S002689331204005X

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