Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors

Abstract

Among the main objectives of biomedical and proteomic research is to identify non-covalent interactions involving proteins. Here we provide a detailed protocol to apply matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry for such a purpose using proteases and protease inhibitors in complex biological samples. Our… (More)
DOI: 10.1038/nprot.2006.487

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@article{Yanes2007DetectionON, title={Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors}, author={Oscar Yanes and Josep Villanueva and Enrique Querol and Francesc X. Avil{\'e}s}, journal={Nature Protocols}, year={2007}, volume={2}, pages={119-130} }