Detection of nitric oxide production in mice by spin-trapping electron paramagnetic resonance spectroscopy.

@article{Komarov1995DetectionON,
  title={Detection of nitric oxide production in mice by spin-trapping electron paramagnetic resonance spectroscopy.},
  author={Andrei M. Komarov and Carol S Lai},
  journal={Biochimica et biophysica acta},
  year={1995},
  volume={1272 1},
  pages={29-36}
}
We describe here a spin-trapping method combined with X-band electron paramagnetic resonance (EPR) spectroscopy for ex vivo measurement of nitric oxide (.NO) levels in the urine of both normal and lipopolysaccharide (LPS)-induced shock mice. Normal or LPS-treated mice were injected subcutaneously with a metal-chelator complex, N-methyl-D-glucamine dithiocarbamate-ferrous iron, [(MGD)2/Fe], which binds to .NO and forms a water-soluble [(MGD)2/Fe-NO] complex. At 2 h after injection of the [(MGD)2… CONTINUE READING

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