Detection of neutralizing antibodies against human T-cell leukemia virus type 1 using a cell-free infection system and polymerase chain reaction.

@article{Haraguchi1994DetectionON,
  title={Detection of neutralizing antibodies against human T-cell leukemia virus type 1 using a cell-free infection system and polymerase chain reaction.},
  author={Yuji Haraguchi and Dong Won Yang and Akiyoshi Handa and Norio Shimizu and Yuetsu Tanaka and Hiroo Hoshino},
  journal={International journal of cancer},
  year={1994},
  volume={59 3},
  pages={416-21}
}
We have developed a cell-free infection system to titrate neutralizing antibodies against human T-cell leukemia virus type 1 (HTLV-1) using the polymerase chain reaction (PCR). S+L-CCC (8C) feline kidney or U-251 MG human glioma cells were infected with a cell-free culture supernatant derived from HTLV-1-infected c77 feline cells. DNA was extracted from 8C or U-251 MG cells after incubation for 24 hr and amplified by PCR. The c77 cell supernatant gave discrete bands, whereas those of HTLV-1… CONTINUE READING

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