Detection of neutral and charged mutations in alpha- and beta-human globin chains by capillary zone electrophoresis in isoelectric, acidic buffers.

Abstract

A simple and reliable method, for screening for point mutations in alpha- and beta-human globin chains, is reported here, utilizing capillary zone electrophoresis in isoelectric, acidic buffers. A solution of 50 mM iminodiacetic acid (pI 2.23) containing 7 M urea and 0.5% hydroxyethylcellulose (apparent pH 3.2) is used as background electrolyte for fast… (More)

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