Detection of natural infection in Lutzomyia cruzi and Lutzomyia forattinii (Diptera: Psychodidae: Phlebotominae) by Leishmania infantum chagasi in an endemic area of visceral leishmaniasis in Brazil using a PCR multiplex assay.

@article{PitaPereira2008DetectionON,
  title={Detection of natural infection in Lutzomyia cruzi and Lutzomyia forattinii (Diptera: Psychodidae: Phlebotominae) by Leishmania infantum chagasi in an endemic area of visceral leishmaniasis in Brazil using a PCR multiplex assay.},
  author={Daniela de Pita-Pereira and Maria Angelica Batista Cardoso and Carlos Roberto Alves and Reginaldo Peçanha Brazil and Constança Fel{\'i}cia De Paoli de Carvalho Britto},
  journal={Acta tropica},
  year={2008},
  volume={107 1},
  pages={66-9}
}
In order to identify Lutzomyia spp. naturally infected by Leishmania parasites a PCR multiplex assay coupled to non-isotopic hybridization was used for the analysis of insect samples collected by CDC light traps in an endemic area of visceral leishmaniasis (VL) in the municipality of Corumbá, Mato Grosso do Sul State, Brazil in May/June 2006. Wild sand flies were identified and grouped into pools of 10 female specimens and 27 groups in total were collected. Positive results were obtained from… CONTINUE READING
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